Tissue Culture Multiple Choice Questions (MCQs) & Answers

Tissue Culture Multiple Choice Questions(MCQs)& Answers Tissue Culture

Tissue Culture Multiple Choice Questions(MCQs)& Answers

CONCEPTS & THEORIES

tissue-culture

About Tissue Culture


Tissue culture is a method of biological research in which fragments of tissue from an animal or plant are transferred to an artificial environment in which they can continue to survive and function. The cultured tissue may consist of a single cell, a population of cells, or a whole or part of an organ.
*Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed.

Cell culture

Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (CO2, O2), and regulates the physio-chemical environment (pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture).

Organ culture

Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions of an organ in various states and conditions by the use of the actual in vitro organ itself. The parts of an organ or a whole organ can be cultured in vitro. The main objective is to maintain the architecture of the tissue and direct it towards normal development. In this technique, it is essential that the tissue is never disrupted or damaged. It thus requires careful handling. The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ culture can be classified into-
(i) those employing a solid medium and
(ii) those employing liquid medium.

Plant tissue culture

Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including-
-The production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits.
-To quickly produce mature plants.
-The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds.
-The regeneration of whole plants from plant cells that have been genetically modified.
-The production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens.
-The production of plants from seeds that otherwise have very low chances of germinating and growing, i.e. orchids and Nepenthes.
-To clean particular plants of viral and other infections and to quickly multiply these plants as ‘cleaned stock’ for horticulture and agriculture.

Culture Medium

Culture Medium or Growth Medium is a solid, liquid or semi-solid designed to support the growth of microorganisms or cells, or small plants like the moss Physcomitrella patens. Different types of media are used for growing different types of cells.
Culture media contain all the elements that most bacteria need for growth and are not selective, so they are used for the general cultivation and maintenance of bacteria kept in laboratory culture collections.

QUESTIONS

Tissue Culture Multiple Choice Questions(MCQs)& Answers

1 Callus is the
A Tissue that forms embryo
B An insoluble carbohydrate
C Tissue that grows to form embryoid
D Un organised actively dividing mass of cells maintained in cultured

Answer: Un organised actively dividing mass of cells maintained in cultured
2 Which of the following is the main effect of cytokines in the tissue culture system?
A Adventitious shoot formation
B Induction of somatic embryos
C Adventitious root formation
D Shoot elongation

Answer: Adventitious shoot formation
3 Which of the following is NOT a plant growth regulator?
A Auxin
B Cytokinins
C Abcisic acid
D Polyphenols

Answer: Polyphenols
4 Growth hormone producing apical dominance is
A Auxin
B Gibberellin
C Ethylene
D Cytokinin

Answer: Auxin
5 Part of plant used for culturing is called
A Scion
B Explant
C Stock
D Callus

Answer: Explant
6 DMSO (Dimethyl sulfoxide) is used as
A Gelling agent
B alkaylating agent
C Chelating agent
D Cryoprotectant

Answer: Cryoprotectant
7 Hormone pair required for a callus to differentiate are
A auxin and cytokinin
B auxin and ethylene
C auxin and absiccic acid
D cytokinins and gibberllin

Answer: auxin and cytokinin
8 Synthetic seed is produced by encapsulating somatic embryo with
A sodium chloride
B sodium alginate
C sodium acetate
D sodium nitrate

Answer: sodium alginate
9 The production of secondary metabolites require the use of
A protoplast
B cell suspension
C meristem
D auxillary buds

Answer: cell suspension
10 Who is the father of tissue culture?
A Bonner
B Haberlandt
C Laibach
D Gautheret

Answer: Haberlandt
11 The concept of invitro cell culture was developed by
A Kotte and Robins
B Haberlandt
C Hanning
D Knop

Answer: Haberlandt
12 Which of the following plant hormone control fruit ripening?
A Ethylene
B Auxin
C Gibbrellins
D Abscisis acid

Answer: Ethylene
13 Which one of them is NOT the main effect of polyamines in the tissue culture system?
A Promotion of tuber and bulb formation
B Adventitious root formation
C Promotion of shoot formation
D Somatic embryogenesis

Answer: Promotion of tuber and bulb formation
14 Which vector is mostly used in crop improvement?
A Plasmid
B Cosmid
C Phasmid
D Agrobacterium

Answer: Agrobacterium
15 Which of the following plant cell will show totipotency?
A Xylem vessels
B Sieve tube
C Meristem
D Cork cells

Answer: Meristem
16 Somaclonal variations are the ones
A Caused by mutagens
B Produce during tissue culture
C Caused by gamma rays
D Induced during sexual embryogeny

Answer: Produce during tissue culture
17 To obtain haploid plant, we culture
A Entire anther
B Nucleus
C Embryo
D Apical bud

Answer: Entire anther
18 A medium which is composed of chemically defined compound is called
A Natural media
B Synthetic media
C Artificial media
D None of these

Answer: Synthetic media
19 The growth of plant tissues in artificial media is called___________
A Gene expression
B Transgenesis
C Plant tissue culture
D Cell hybridization

Answer: Plant tissue culture
20 Which of the following is NOT the feature of plant cells?
A Presence of centrioles
B The cell wall outside the cell membrane
C Cell-cell communication through plasmodesmata
D Consists of plastids

Answer: Presence of centrioles
21 Name the term given to the ability of single cells to divide and produce all the differentiated cell in the organism?
A Totipotency
B Multipotent
C Unipotent
D Pluripotent

Answer: Totipotency
22 Out of the following, which one is NOT the basic component of culture media used for plant cultivation?
A Complex mixture of salts
B Amino acids
C Serum albumin
D Sugar/ sucrose

Answer: Serum albumin
23 Mark the INCORRECT statement about agar, a gelling agent in plant tissue culture medium?
A Not digested by plant enzymes
B It does not use in microprapogation work
C It does not react with media constituents
D Remain stable at incubation temperature

Answer: It does not use in microprapogation work
24 DNA is separated by
A Agarose gel electrophoresis
B PAGE
C SDS-PAGE
D SDA-PAGE

Answer: Agarose gel electrophoresis
25 Polymerase Chain Reaction involves
A Denaturation, annealing and polymerisation
B Denaturation and polymerisation
C extension and annealing
D None of these

Answer: Denaturation, annealing and polymerisation
26 The term ‘pharming’ refers to
A Genetically modified foods from plants
B Drugs synthesis from transgenic plants
C recombinant drugs from bacteria
D transgenic research by animals

Answer: Drugs synthesis from transgenic plants
27 The first report of forming haploid embryos from Datura by invitro was published
A Nitch
B Guha and Maheswary
C Maheswary
D Bourgin and Nitch

Answer: Guha and Maheswary
28 The embryo formed by unfertilized egg
A Androgenic embryos
B somatic embryos
C parthenogenic embryos
D adventive embryos

Answer: parthenogenic embryos
29 Cybrids are produced by
A Fusion of two different nuclei from two different species
B Fusion of two same nuclei from same species
C Nucleus of one species but cytoplasm from both the parent species
D None of the above

Answer: Nucleus of one species but cytoplasm from both the parent species
30 The most widely used chemical for protoplast fusion, as fusogens, is
A Manitol
B Sorbitol
C Mannol
D Poly ethylene glycol (PEG)

Answer: Poly ethylene glycol (PEG)

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