In the other post we already discussed about Chromatography MCQ and Answer; MCQ On Paper Chromatography and others. These MCQ On Gel Filtration Chromatography are most important for Biochemistry, B.Sc/M.Sc/Phd Botany/Chemistry, Biology, Chemistry. These MCQ On Gel Filtration Chromatography helps you to improve your conceptual insight on the phase system.
MCQ On Gel Filtration Chromatography
1. In gel filtration chromatography, separation of proteins are based on their
a) size and net charge
b) shape and net charge
c) size and specific affinity
d) size and shape
Answer: d
2. For gel filtration chromatography of proteins, which of the following is True?
a) Large or elongated proteins enter the pores in the beads.
b) Small proteins enter the pores in the beads.
c) Large or elongated proteins elute from the bottom of the column later.
d) Small proteins elute from the bottom of the column first.
Answer: b
3. For gel filtration column chromatography, which two of the following has linear relationship?
i. Amount of protein.
ii. Relative elution volume of a protein (void volume over elution volume of a protein.
iii. Logarithm of protein molecular mass.
a) i and ii
b) ii and iii
c) i and iii
d) None of these
Answer: b
4. Which of the following are stationary phases commonly used for the gel filtration column?
i. Dextran
ii. Agarose
iii. Silica
a) i and ii only
b) ii and iii only
c) i and iii only
d) All the them
Answer: d
5. Which of the following is/are another term of gel filtration?
i. Desalting
ii. Size exclusion chromatography
iii. Permeation chromatography
iv. Molecular sieve chromatography
a) i and ii only
b) i and iii only
c) ii and iii only
d) ii, iii and iv only
Answer: d
6. Which of the following is not the application of gel filtration?
a) Purification
b) Relative molecular mass determination
c) Protein concentration
d) None of these
Answer: d
7. The use of insulin hormone to purify its receptor is an example of
a) Ion exchange chromatography
b) Affinity chromatography
c) Gel filtration chromatography
d) Ligand mediated chromatography
Answer: b