Are you looking for Processes of Recombinant DNA Technology MCQs and Answers for medical entrance examinations? If yes, these are some most important multiple choice questions and answers on NCERT Class-12 Biology, Chapter-11 (Microbes in Human Welfare).
Here we composed these Processes of Recombinant DNA Technology MCQs and Answers after reading the chapter on Biotechnology Principles and Processes from the NCERT book on Class 12 Biology. The questions are topics-wise and the students can relate the questions easily to the most important topics of this chapter.
Processes of Recombinant DNA Technology MCQs and Answers
1. The nucleic acid in most the organisms is ______
2. The bacterial cells can be lysed by using ______ enzyme.
3. The plant cells can be lysed by using ______ enzyme.
4. The fungal cells can be lysed by using ______ enzyme.
5. The enzyme which cleaves DNA is _______
6. The restriction enzyme needs to be in _____ form to cut the DNA.
7. Which macromolecules are present along with DNA within the cell?
c) Vectors, organelles
d) Lipids, polysaccharides
8. ______ is a monomer of lipids.
a) Amino acid
c) Fatty acids
9. ______ is the monomer of proteins.
b) Fatty acids
c) Amino acid
10. _______ is the building block of carbohydrates.
b) Amino acid
d) Fatty acids
11. The enzyme which cleaves RNA is _______
12. The antibiotic resistance gene can be used as ________ marker for selecting transformants.
13. The common bacteria used in genetic engineering is _____
14. Recombinant DNA technology is associated with _______
a) Christian Gram
b) C. Darwin
c) Robert Koch
d) Herbert Boyer
15. If any protein-encoding gene is expressed in a heterologous host, it is called a _______ protein.
16. Which culture system is used to obtain cells in the exponential phase?
17. _______ culturing method produces higher biomass and higher yield of the desired product.
18. ________ growth conditions are required to obtain the maximum yield.
19. How many stages does a bacterial growth curve have?
20. What facilitates the even mixing of ingredients within a bioreactor?
21. Downstream processing also involves _________
a) separation and purification
b) mixing and hybridization
c) separation and hybridization
d) mixing and purification
22. Log phase is also known as _________
a) lag phase
b) exponential phase
c) stationary phase
d) decline phase
23. The enzyme which cleaves proteins is ______
24. Which protein helps in DNA winding?
25. Which chemical is used to obtain a precipitate of pure DNA?
26. DNA is _____ charged.
27. DNA moves towards ______ during agarose gel electrophoresis.
a) negative terminal
d) does not move
28. Which enzyme helps in joining of DNA?
29. What does PCR stand for?
a) Polymerase chain radioactivity
b) Polymerase chitin reaction
c) Phenol carbohydrate reaction
d) Polymerase chain reaction
30. The three steps involved in PCR are ________
a) annealing, extension, and denaturation
b) extension, denaturation, and annealing
c) denaturation, annealing, and extension
d) denaturation, extension, and annealing
31. Taq polymerase is obtained from _______
c) Thermus aquaticus
32. The transformants containing ______ gene will grow on plates containing antibiotics.
b) amino acid
c) selectable marker
33. Bioreactors can contain volume up to ______ litres.
d) 50Answer: b
FAQs on Processes of Recombinant DNA Technology
Q1. What is recombinant DNA technology?
Answer: Recombinant DNA technology is a process by which DNA from two different sources is combined to create a new DNA molecule. This new molecule can be used to express a desired trait or characteristics.
Q2. What are the steps involved in recombinant DNA technology?
Answer: There are four main steps involved in recombinant DNA technology:
1. Isolation of the DNA: This involves isolating the DNA from the cells that you want to use. This can be done using a number of methods, such as centrifugation or chromatography.
2. Cutting of the DNA: The next step is to cut the DNA into smaller pieces using enzymes called restriction enzymes.
3. Joining of the DNA: The DNA pieces are then joined together using another enzyme called DNA ligase.
4. Expression of the DNA: The final step is to express the desired trait or characteristics by introducing the recombinant DNA into host cells. This can be done using a number of methods, such as transformation or transfection.