Tools of Recombinant DNA Technology: Restriction Enzymes MCQs and Answers

Are you looking for Tools of Recombinant DNA Technology: Restriction Enzymes MCQs and Answers for medical entrance examinations? If yes, these are some most important multiple choice questions and answers on NCERT Class-12 Biology, Chapter-11 (Microbes in Human Welfare).

Here we composed these Tools of Recombinant DNA Technology: Restriction Enzymes MCQs and Answers after reading the chapter on Biotechnology Principles and Processes from the NCERT book on Class 12 Biology. The questions are topics-wise and the students can relate the questions easily to the most important topics of this chapter.

Tools of Recombinant DNA Technology: Restriction Enzymes MCQs and Answers

1. From which organism was the first restriction enzyme isolated?

a) Escherichia coli

b) Salmonella typhimurium

c) Bacillus cereus

d) Staphylococcus aureus

Answer: a


2. In genetic engineering, restriction enzymes cleave the DNA at a specific site known as _____

a) restriction

b) recognition

c) promoter

d) sense

Answer: b


3. Restriction enzymes are also known as _________

a) ligase

b) polymerase

c) telomerase

d) restriction endonucleases

Answer: d


4. _____ was the first restriction endonuclease was isolated and characterized.

a) EcoRI

b) BamHI

c) Hind II

d) Sma I

Answer: c


5. Restriction enzymes belong to a larger class of enzymes called ______

a) proteins

b) proenzyme

c) nucleases

d) isozymes

Answer: c


6. The _______ in DNA are base pair sequences that are the same when read forward or backward from a central axis of symmetry.

a) nucleases

b) palindrome

c) termination sequences

d) amino acids

Answer: b


7. Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______

a) polymerase chain reaction

b) gene amplification

c) blotting

d) gel electrophoresis

Answer: d


FAQs on Tools of Recombinant DNA Technology: Restriction Enzymes

Q1. What are restriction enzymes?

Answer: Restriction enzymes are enzymes that cut DNA at specific recognition sites. recognition sites are typically 4-8 base pairs long and are located within a chromosome.

Q2. Why are restriction enzymes important?

Answer: Restriction enzymes are important because they are used in a variety of techniques, including DNA cloning, PCR, and sequencing.

Q3. What are some of the most commonly used restriction enzymes?

Answer: Some of the most commonly used restriction enzymes are EcoRI, BamHI, and HindIII.

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