Are you looking for Tools of Recombinant DNA Technology: Restriction Enzymes MCQs and Answers for medical entrance examinations? If yes, these are some most important multiple choice questions and answers on NCERT Class-12 Biology, Chapter-11 (Microbes in Human Welfare).
Here we composed these Tools of Recombinant DNA Technology: Restriction Enzymes MCQs and Answers after reading the chapter on Biotechnology Principles and Processes from the NCERT book on Class 12 Biology. The questions are topics-wise and the students can relate the questions easily to the most important topics of this chapter.
Tools of Recombinant DNA Technology: Restriction Enzymes MCQs and Answers
1. From which organism was the first restriction enzyme isolated?
a) Escherichia coli
b) Salmonella typhimurium
c) Bacillus cereus
d) Staphylococcus aureus
Answer: a
2. In genetic engineering, restriction enzymes cleave the DNA at a specific site known as _____
a) restriction
b) recognition
c) promoter
d) sense
Answer: b
3. Restriction enzymes are also known as _________
a) ligase
b) polymerase
c) telomerase
d) restriction endonucleases
Answer: d
4. _____ was the first restriction endonuclease was isolated and characterized.
a) EcoRI
b) BamHI
c) Hind II
d) Sma I
Answer: c
5. Restriction enzymes belong to a larger class of enzymes called ______
a) proteins
b) proenzyme
c) nucleases
d) isozymes
Answer: c
6. The _______ in DNA are base pair sequences that are the same when read forward or backward from a central axis of symmetry.
a) nucleases
b) palindrome
c) termination sequences
d) amino acids
Answer: b
7. Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______
a) polymerase chain reaction
b) gene amplification
c) blotting
d) gel electrophoresis
Answer: d
FAQs on Tools of Recombinant DNA Technology: Restriction Enzymes
Q1. What are restriction enzymes?
Answer: Restriction enzymes are enzymes that cut DNA at specific recognition sites. recognition sites are typically 4-8 base pairs long and are located within a chromosome.
Q2. Why are restriction enzymes important?
Answer: Restriction enzymes are important because they are used in a variety of techniques, including DNA cloning, PCR, and sequencing.
Q3. What are some of the most commonly used restriction enzymes?
Answer: Some of the most commonly used restriction enzymes are EcoRI, BamHI, and HindIII.
Categories: Biology Class-12